Comparative Transcriptome Analysis of Anthocyanin Biosynthesis in Pansy (Viola × wittrockiana Gams.)

Pansy (Viola × wittrockiana Gams.) is an important and attractive ornamental plant with a wide variety of flower colors. To date, the molecular genetic understanding its colorful petal pigment patterns remains largely unknown. In this study, we analyzed bicolor petals “Mengdie” in cytological, physiological, transcriptomic aspects. Results showed that difference colors was mainly determined by distribution epidermal cells. Pigment analysis indicated anthocyanins had strong correlations color parameters, which acted as main factor coloration. Comparative transcriptome found total 43,908 unigenes mean length 682 bp. There were 24,323, 16,668, 8507, 7680 annotated Nr, Swiss-Prot, KOG, KEGG databases, respectively. Differential expression genes (DEGs) anthocyanin late biosynthesis (LBGs), VwF3′H, VwF3′5′H, VwUFGT, would be likely to play major role formation pansy. The selected DEGs verified qRT-PCR. This study contributes excellent insight into mechanism provides some useful information for modification